Cell wall of feeds and their impact on protein digestibility: An in vitro method applied for pig nutrition

Plant-derived by-products of the food, drink, and fuel industry are low-cost ingredients, that routinely used to formulate feeds for livestock, especially pigs. Often, those ingredients rich in dietary fibres (DF), which is known have a negative influence on digestive use nutrients The effect DF nutrient bioaccessibility (i.e., release from plant tissue) digestion transformation tissue during hydrolysis by enzymes) depends their amount, but more importantly, nature organisation within cell wall. aim this work was determine how different sources affected protein proteolysis. We hypothesised released feed matrix pig depend mainly amount wall polysaccharides fibre). To test this, we conducted an vitro study where nine commonly feeding soybean meal, barley, wheat, two varieties peas, fava bean, chickpea, maize sugar beet pulp) were digested using simulated gastrointestinal model. Cell integrity proteolysis assessed biochemical, biophysical, microscopic techniques. Protein differed among (P < 0.01), with pulp having lowest fraction (about 34 %) chickpea greatest (93 %). values also varied greatly 0.01) did not always follow same trend as bioaccessibility. For instance, meal had highest 96 % 50 %. particle size distribution microscopy images provide explanation these differences, related physical barrier, swelling, rupture This illustrated structure specific each ingredient, consequences digestibility cannot be characterised composition alone. In formulation, concept ought accounted achieve optimal uptake animal.